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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. |
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Título: |
Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 321. |
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Série: |
(Embrapa Soja. Documentos, 228).
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
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Conteúdo: |
It is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression. MenosIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this m... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03691naa a2200229 a 4500
001 1466852
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aFUGANTI, R.
245 $aMicrosatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica.
260 $c2004
300 $ap. 321.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression.
700 1 $aBENEVENTI, M. A.
700 1 $aSILVA, J. F. V.
700 1 $aARIAS, C. A. A.
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
20/01/2009 |
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Data da última atualização: |
20/01/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GAMA, J. R. V.; BENTES-GAMA, M. de M. |
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Afiliação: |
João Ricardo Vasconcelos Gama, UFRA; Michelliny de Matos Bentes-Gama, Embrapa Rondônia. |
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Título: |
Exploração tradicional madeireira em floresta de várzea por comunidades ribeirinhas do estuário amazônico na ilha de Marajó, Brasil. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESO FORESTAL LATINOAMERICANO, 4., 2008, Mérida, Venezuela. Hacia el ordenamiento territorial y el manejo sustentable de los bosques: libro de resúmenes. [S.l.: s. n.], 2008. |
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Páginas: |
p. 125-126. |
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Idioma: |
Português |
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Notas: |
Meta 2008. |
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Conteúdo: |
O objetivo deste trabalho foi caracterizar a exploração madeireira tradicional realizada por comunidades ribeirinhas no município de Afuá. |
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Palavras-Chave: |
Comunidades tradicionais; Manejo Florestal; Produto florestal não madeireiro. |
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Thesagro: |
Euterpe Oleracea. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00940naa a2200205 a 4500
001 1700865
005 2009-01-20
008 2008 bl --- 0-- u #d
100 1 $aGAMA, J. R. V.
245 $aExploração tradicional madeireira em floresta de várzea por comunidades ribeirinhas do estuário amazônico na ilha de Marajó, Brasil.
260 $c2008
300 $ap. 125-126.
500 $aMeta 2008.
520 $aO objetivo deste trabalho foi caracterizar a exploração madeireira tradicional realizada por comunidades ribeirinhas no município de Afuá.
650 $aEuterpe Oleracea
653 $aComunidades tradicionais
653 $aManejo Florestal
653 $aProduto florestal não madeireiro
700 1 $aBENTES-GAMA, M. de M.
773 $tIn: CONGRESO FORESTAL LATINOAMERICANO, 4., 2008, Mérida, Venezuela. Hacia el ordenamiento territorial y el manejo sustentable de los bosques: libro de resúmenes. [S.l.: s.$gn.], 2008.
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